Jefferson Pike
- Direction:
 - North/South
 - Southern Terminus:
 - West Virginia-Virginia state line
 - Northern Terminus:
 - Virginia-Maryland state line
 - Distance:
 - 0.57 miles
 - Counties:
 - Loudoun
 - Signed:
 - Yes
 
US 340 is a north/south route extending from Greenville, Virginia, to Frederick, Maryland. After passing through the eastern panhandle of West Virginia, it re-enters Virginia for a very short distance before crossing the Potomac River to Maryland. This northern section, only about half a mile long, is known as Jefferson Pike and has a single intersection before leaving the state again.
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